Technical Notes
Improve and optimize your cell culture
Physioxia for Cell Culture
If you’ve worked with cell cultures, you’re probably familiar with the term ‘normoxia,’ which refers to the standard 21% oxygen level used in many labs. But did you know that most cells in the human body actually experience much lower oxygen levels, typically between 1 to 8%? This is called ‘physioxia,’ or physiologic oxygen. This video explores why mimicking these conditions can significantly improve your cell culture outcomes.
Read More
Optimizing In Vitro Fertilization Outcomes: The Role of Low Oxygen Conditions in Embryo Culture
Learn about the benefits and implementation of physioxic low oxygen conditions in embryo culture to optimize in vitro fertilization (IVF) outcomes.
Differential Regulation of Autophagy Under Hypoxic, Physioxic, and Normoxic Conditions
Explore how autophagy is differentially regulated under varying oxygen levels.
Immune Cells in Their Element: The Critical Role of Physiological Oxygen
Physiological oxygen is a fundamental factor shaping immune cell metabolism, signaling, activation, and effector functions.
Effects of Hypoxia and Physioxia on the Metabolome
Learn how varying oxygen levels, specifically hypoxia and physioxia, impact cellular metabolism by altering pathways related to energy production, redox balance, biosynthesis, and signaling.
Optimization of Physioxic Organoid Cell Culture for Research and Drug Discovery
Improve your organoid cultures by understanding how low oxygen better replicates the in vivo microenvironment, enhances stem cell maintenance, and improves the functionality and maturation of organoids.
Adoptive Cell Therapies and Low Oxygen
Watch to uncover the potential of low oxygen in optimizing adoptive cell therapies.
Watch Video
Physiological Oxygen for Neuronal Cell Culture
Explore the differences in neuronal cell behavior under low oxygen (physiological) compared to atmospheric oxygen conditions and gain insights into optimizing neuronal cell culture systems.
Stem Cell Culture Requires Low Oxygen
Explore the importance of hypoxia in stem cell culture, the molecular pathways involved, and its implications for optimizing stem cell applications.
Malaria Research and Therapeutic Discovery
A quick summary on the latest key points for malaria research and discovery.
Read More
Epigenetics and Hypoxia
Watch to learn how hypoxia drives epigenetic changes leading to cellular changes.
Watch Video
Hypoxia and the Tumor Microenvironment
Discover how the hypoxic tumor microenvironment fuels cancer growth.
Watch Video
Key Advances in Cellular Hypoxia Discovery
Learn about the key advancements that have driven our understanding of cellular responses to hypoxia in this infographic timeline.
Read More
Cellular Transcriptome and Proteome Dynamics Under Hypoxia
Hypoxia can induce mass transcriptomic and proteomic changes across many different cell types, supporting the concept that the status of cells at normoxic conditions are not the same as those at physiologic or low oxygen.
MIC-101 advantages over standard incubators
The Modular Incubator Chamber (MIC-101) has a set of unique characteristics that provide distinct advantages over standard continuous flow CO2 incubators.
Read More
A summary of published physioxic cell culture conditions
Check out our table summarizing data on published physioxic conditions tested for various cell lines and primary cells and the experimental outcomes.
Read More
Is oxygen sabotaging your research?
Studies clearly show that cells cultured under appropriate physiological conditions express regulator genes in vivo that are silent under normoxic (21% O2) concentration.
Read More
Eliminate the Edge Effect
Evaporation of media in multi-well plates, placed in traditional CO2 incubators, can exceed 15 to 25% in outside and corner wells, respectively!
Read More
HIF-1α discovery,
function, and the 2019 Nobel Prize
Learn more about the background and importance of Hypoxia-Inducible Factor 1 (HIF-1) in regulating functions across many cell types.
Read MoreJoin our eNewsletter
Stay up-to-date with our latest technical notes, announcements, and new products.