Technical Notes

Hypoxia and the Tumor Microenvironment

Discover how the hypoxic tumor microenvironment fuels cancer growth.

Key Advances in Cellular Hypoxia Discovery

Learn about the key advancements that have driven our understanding of cellular responses to hypoxia.

Cellular Transcriptome and Proteome Dynamics Under Hypoxia

Hypoxia can induce mass transcriptomic and proteomic changes across many different cell types, supporting the concept that the status of cells at normoxic conditions are not the same as those at physiologic or low oxygen.

MIC-101 advantages over standard incubators

The Modular Incubator Chamber (MIC-101) has a set of unique characteristics that provide distinct advantages over standard continuous flow CO₂ incubators.

A summary of published physioxic cell culture conditions

Check out our table summarizing data on published physioxic conditions tested for various cell lines and primary cells and the experimental outcomes.

When should you culture cells at 21% oxygen?

The answer is: never. Learn more about why.

Is oxygen sabotaging your research?

Studies clearly show that cells cultured under appropriate physiological conditions express regulator genes in vivo that are silent under normoxic (21% O₂) concentration.

Eliminate the Edge Effect

Evaporation of media in multi-well plates, placed in traditional CO₂ incubators, can exceed 15 to 25% in outside and corner wells, respectively!

HIF-1α discovery, function, and the 2019 Nobel Prize

Learn more about the background and importance of Hypoxia-Inducible Factor 1 (HIF-1) in regulating functions across many cell types.